Hotspot

ndhF-rpl32

SSC · 113151-113830 · 680 bp

Back to Sophora

Pi 0.0356

MarkerSeek score 77.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 680
variable_sites: 69
pi: 0.0356
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCAACGGAACCGCAAAATAA	TTCGAACCGAGATGCTCAAG	45.0	50.0	57.9	58.0	1862-2176 bp	1.0	90.3
ndhF-rpl32_p2	GCAACGGAACCGCAAAATAA	AAATAAAATGCCATGCCGCC	45.0	45.0	57.9	58.0	1889-2203 bp	1.0	89.8
ndhF-rpl32_p3	AACAAGAGGGCACACAAAGT	TTCGAACCGAGATGCTCAAG	45.0	50.0	57.9	58.0	1844-2158 bp	1.0	89.6
ndhF-rpl32_p4	GCAACGGAACCGCAAAATAA	AAGCACTGCTTCCTAAGAGC	45.0	50.0	57.9	57.9	1845-2159 bp	1.0	89.4
ndhF-rpl32_p5	GCAACGGAACCGCAAAATAA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.9	57.9	1835-2149 bp	1.0	89.3