Hotspot

rps11-rpl36

LSC · 79587-80010 · 424 bp

Back to Sophora

Pi 0.0558

MarkerSeek score 73.20

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 424
variable_sites: 74
pi: 0.0558
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	TTCCACTGTAGTGTCCGAGT	CACTTCCCAAGGGTCTGTTC	50.0	55.0	58.0	58.1	1362-1467 bp	1.0	90.2
rps11-rpl36_p2	ACTTCCACTGTAGTGTCCGA	AGGATTCGACTCGACGTACT	50.0	50.0	58.0	58.0	1287-1320 bp	0.888889	85.9
rps11-rpl36_p3	TTCCACTGTAGTGTCCGAGT	AGGATTCGACTCGACGTACT	50.0	50.0	58.0	58.0	1285-1318 bp	0.888889	85.8
rps11-rpl36_p4	CACTTCCACTGTAGTGTCCG	AGGATTCGACTCGACGTACT	55.0	50.0	57.9	58.0	1288-1321 bp	0.888889	85.4
rps11-rpl36_p5	TCTCCGAGCAAAACTCTTCG	AGGATTCGACTCGACGTACT	50.0	50.0	57.9	58.0	1405-1438 bp	0.888889	85.0