Hotspot

trnP-GGG-psaJ

LSC · 66460-66992 · 533 bp

Back to Sophora

Pi 0.0451

MarkerSeek score 80.10

Primer available yes

Label trnP-GGG-psaJ

Feature Metadata

label_name: trnP-GGG-psaJ
feature_type: igs
region: LSC
length_bp: 533
variable_sites: 72
pi: 0.0451
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-GGG-psaJ_p1	CGAACTGAACTAAGAGCGCT	TAATATTGTTGCCTCCGCCC	50.0	50.0	58.0	58.0	1226-1273 bp	1.0	91.7
trnP-GGG-psaJ_p2	CGAACTGAACTAAGAGCGCT	TTCTTCAACCCTTCCTGTGC	50.0	50.0	58.0	58.0	947-1009 bp	1.0	91.5
trnP-GGG-psaJ_p3	ATCGACGTGGTGATCAGTTG	TAATATTGTTGCCTCCGCCC	50.0	50.0	58.0	58.0	1454-1466 bp	0.666667	74.9
trnP-GGG-psaJ_p4	ATCGACGTGGTGATCAGTTG	TTTACCCTTGGCCATGAACC	50.0	50.0	58.0	58.1	1603-1615 bp	0.666667	74.4
trnP-GGG-psaJ_p5	ATCGACGTGGTGATCAGTTG	TTCTTCAACCCTTCCTGTGC	50.0	50.0	58.0	58.0	1175-1195 bp	0.666667	73.5