Hotspot

psbZ-trnG-GCC

LSC · 45294-45689 · 396 bp

Back to Sophora

Pi 0.0628
MarkerSeek score 82.30
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
396
variable_sites
65
pi
0.0628
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 AGTGGGTATTCTTAATTCTCTCA TACCATTCGACTATATCCGC 34.8 45.0 54.5 54.3 445-547 bp 1.0 50.9
psbZ-trnG-GCC_p2 AGTGGGTATTCTTAATTCTCTCATCT TACCATTCGACTATATCCGC 34.6 45.0 57.4 54.3 445-547 bp 1.0 50.9
psbZ-trnG-GCC_p3 AGTGGGTATTCTTAATTCTCTCA TTACCATTCGACTATATCCGC 34.8 42.9 54.5 55.1 446-548 bp 1.0 50.9
psbZ-trnG-GCC_p4 GTCTTTCTAGTGGGTATTCTT TACCATTCGACTATATCCGC 38.1 45.0 52.3 54.3 453-555 bp 1.0 50.9
psbZ-trnG-GCC_p5 AGTGGGTATTCTTAATTCTCTCATCT TTACCATTCGACTATATCCGC 34.6 42.9 57.4 55.1 446-548 bp 1.0 50.9