Hotspot

trnT-UGU-trnL-UAA

LSC · 34617-35894 · 1278 bp

Back to Sophora

Pi 0.0572
MarkerSeek score 82.70
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
1278
variable_sites
185
pi
0.0572
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.97
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 AGGTCCTCTGTAACGTGACA TAGCGTCTACCAATTTCGCC 50.0 50.0 58.0 58.1 1607-1689 bp 1.0 91.4
trnT-UGU-trnL-UAA_p2 AGGTCCTCTGTAACGTGACA TCTCGCTTTATGCTCGCTTT 50.0 45.0 58.0 58.0 1760-1829 bp 0.555556 70.3
trnT-UGU-trnL-UAA_p3 AGGTCCTCTGTAACGTGACA TTCTCGCTTTATGCTCGCTT 50.0 45.0 58.0 58.0 1761-1830 bp 0.555556 70.3
trnT-UGU-trnL-UAA_p4 CGAAGCCATACCCAATCGAA TCTCGCTTTATGCTCGCTTT 50.0 45.0 58.1 58.0 2051-2120 bp 0.555556 69.5
trnT-UGU-trnL-UAA_p5 CGAAGCCATACCCAATCGAA TTCTCGCTTTATGCTCGCTT 50.0 45.0 58.1 58.0 2052-2121 bp 0.555556 69.5