Hotspot

trnG-UCC-trnS-GCU

LSC · 32596-33329 · 734 bp

Back to Sophora

Pi 0.0577
MarkerSeek score 77.10
Primer available yes
Label trnG-UCC-trnS-GCU

Feature Metadata

label_name
trnG-UCC-trnS-GCU
feature_type
igs
region
LSC
length_bp
734
variable_sites
96
pi
0.0577
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.95
markerseek_score
77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnS-GCU_p1 ACGAATCGCACTTTTACCACT CAATCCGACGCTTTAGTCCA 42.9 50.0 58.2 58.0 204-806 bp 1.0 82.1
trnG-UCC-trnS-GCU_p2 ACGAATCGCACTTTTACCACT AATCCGACGCTTTAGTCCAC 42.9 50.0 58.2 58.0 203-805 bp 1.0 82.1
trnG-UCC-trnS-GCU_p3 ACGAATCGCACTTTTACCAC CAATCCGACGCTTTAGTCCA 45.0 50.0 56.8 58.0 204-806 bp 1.0 82.0
trnG-UCC-trnS-GCU_p4 ACGAATCGCACTTTTACCAC AATCCGACGCTTTAGTCCAC 45.0 50.0 56.8 58.0 203-805 bp 1.0 82.0
trnG-UCC-trnS-GCU_p5 TCCATAGAACGAATCGCACT CAATCCGACGCTTTAGTCCA 45.0 50.0 56.8 58.0 212-814 bp 1.0 82.0