Hotspot

trnV-UAC-ndhC

LSC · 10464-10852 · 389 bp

Back to Sophora

Pi 0.0559

MarkerSeek score 74.20

Primer available yes

Label trnV-UAC-ndhC

Feature Metadata

label_name: trnV-UAC-ndhC
feature_type: igs
region: LSC
length_bp: 389
variable_sites: 54
pi: 0.0559
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-ndhC_p1	AAAGGGTTGAGACGCCAAAT	GCCTAGGACTAGATCGTGGT	45.0	55.0	58.0	58.0	1506-1508 bp	0.333333	53.2
trnV-UAC-ndhC_p2	AAAGGGTTGAGACGCCAAAT	TGCCTAGGACTAGATCGTGG	45.0	55.0	58.0	58.0	1507-1509 bp	0.333333	53.2
trnV-UAC-ndhC_p3	AAAGGGTTGAGACGCCAAAT	TTCATAGTTACTGTGCCCGC	45.0	50.0	58.0	58.0	1546-1548 bp	0.333333	53.2
trnV-UAC-ndhC_p4	AAAGGGTTGAGACGCCAAAT	AGAGAAGCGGCCATAAACTG	45.0	50.0	58.0	58.0	1410-1412 bp	0.333333	53.1
trnV-UAC-ndhC_p5	AAAGGGTTGAGACGCCAAAT	TACATCGAAGCTCATTGCCC	45.0	50.0	58.0	58.0	1155-1157 bp	0.333333	53.0