Hotspot

trnK-UUU-rbcL

LSC · 4377-5314 · 938 bp

Back to Sophora

Pi 0.0551

MarkerSeek score 84.00

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: LSC
length_bp: 938
variable_sites: 135
pi: 0.0551
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	AGCTTTTCCCACATCAAGCA	AATGAGGGACGTGATCTTGC	45.0	50.0	58.0	58.0	1153-1505 bp	1.0	91.8
trnK-UUU-rbcL_p2	AGCTTTTCCCACATCAAGCA	GCCGTAGCTAATCGAGTAGC	45.0	55.0	58.0	58.0	1198-1550 bp	1.0	91.7
trnK-UUU-rbcL_p3	AGCTTTTCCCACATCAAGCA	AGTCCTGAATTAGCTGCTGC	45.0	50.0	58.0	58.0	1093-1445 bp	1.0	91.7
trnK-UUU-rbcL_p4	AGCTTTTCCCACATCAAGCA	GAGGCTAGCAAATGGAGTCC	45.0	55.0	58.0	58.0	1108-1460 bp	1.0	91.7
trnK-UUU-rbcL_p5	AGCTTTTCCCACATCAAGCA	GCTTGTGAAGTGTGGAAGGA	45.0	50.0	58.0	58.0	1376-1427 bp	0.888889	86.4