Hotspot

ycf1

SSC · 124610-130201 · 5592 bp

Back to Sonneratia

Pi 0.00319

MarkerSeek score 45.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5592
variable_sites: 54
pi: 0.00319
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGATCTTTCCACGCTGGC	CATATCCGCCCAGTTTGTCA	61.1	50.0	58.5	58.0	288 bp	1.0	62.2
ycf1_p2	GGATCTTTCCACGCTGGC	ACATATCCGCCCAGTTTGTC	61.1	50.0	58.5	58.0	289 bp	1.0	62.2
ycf1_p3	GGATCTTTCCACGCTGGC	CGCCCAGTTTGTCAACTTTC	61.1	50.0	58.5	57.9	282 bp	1.0	61.6
ycf1_p4	GGATCTTTCCACGCTGGC	TATCCGCCCAGTTTGTCAAC	61.1	50.0	58.5	58.2	286 bp	1.0	61.1
ycf1_p5	GATCTTTCCACGCTGGCG	CATATCCGCCCAGTTTGTCA	61.1	50.0	58.9	58.0	287 bp	1.0	59.0