Hotspot

rbcL

LSC · 57558-59006 · 1449 bp

Back to Sonneratia

Pi 0.00118

MarkerSeek score 51.60

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1449
variable_sites: 4
pi: 0.00118
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TCGAGTAGACCTTGTTGTTGT	AGGATCTTTGTGTTCGGCTC	42.9	50.0	56.9	57.9	1581 bp	1.0	62.7
rbcL_p2	CGAGTAGACCTTGTTGTTGTG	AGGATCTTTGTGTTCGGCTC	47.6	50.0	56.9	57.9	1580 bp	1.0	62.4
rbcL_p3	TCGAGTAGACCTTGTTGTTGT	CAGGATCTTTGTGTTCGGCT	42.9	50.0	56.9	58.2	1582 bp	1.0	62.0
rbcL_p4	TCGAGTAGACCTTGTTGTTGT	GGATCTTTGTGTTCGGCTCA	42.9	50.0	56.9	58.2	1580 bp	1.0	62.0
rbcL_p5	CGAGTAGACCTTGTTGTTGTG	CAGGATCTTTGTGTTCGGCT	47.6	50.0	56.9	58.2	1581 bp	1.0	61.7