Hotspot

trnL-UAG-ccsA

SSC · 114823-114916 · 94 bp

Back to Sonneratia

Pi 0.0208

MarkerSeek score 56.10

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 94
variable_sites: 5
pi: 0.0208
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GTGAAATCGGTAGACACGCT	ACAATTGAAACGATCGAAAAGGA	50.0	34.8	58.0	57.3	224-230 bp	1.0	53.3
trnL-UAG-ccsA_p2	TGAAATCGGTAGACACGCTG	ACAATTGAAACGATCGAAAAGGA	50.0	34.8	58.0	57.3	223-229 bp	1.0	53.3
trnL-UAG-ccsA_p3	TAGACACGCTGCTCTTAGGA	ACAATTGAAACGATCGAAAAGGA	50.0	34.8	57.9	57.3	214-220 bp	1.0	52.5
trnL-UAG-ccsA_p4	GGTAGACACGCTGCTCTTAG	ACAATTGAAACGATCGAAAAGGA	55.0	34.8	57.8	57.3	216-222 bp	1.0	51.9
trnL-UAG-ccsA_p5	GTAGACACGCTGCTCTTAGG	ACAATTGAAACGATCGAAAAGGA	55.0	34.8	57.8	57.3	215-221 bp	1.0	51.9