Hotspot

accD-psaI

LSC · 59815-61930 · 2116 bp

Back to Sonneratia

Pi 0.00199

MarkerSeek score 48.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 2116
variable_sites: 14
pi: 0.00199
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TGGTGGTTCAATTCGATGTTG	CCATTGCAATTGCCGGAAAT	42.9	45.0	57.1	58.0	2223-2229 bp	1.0	65.3
accD-psaI_p2	GGTGGTTCAATTCGATGTTGT	CCATTGCAATTGCCGGAAAT	42.9	45.0	57.1	58.0	2222-2228 bp	1.0	65.3
accD-psaI_p3	TGGTGGTTCAATTCGATGTTG	AAGAAGCCATTGCAATTGCC	42.9	45.0	57.1	57.9	2229-2235 bp	1.0	64.7
accD-psaI_p4	GGTGGTTCAATTCGATGTTGT	AAGAAGCCATTGCAATTGCC	42.9	45.0	57.1	57.9	2228-2234 bp	1.0	64.7
accD-psaI_p5	TCGATGTTGTCTAAGGGGAA	CCATTGCAATTGCCGGAAAT	45.0	45.0	55.9	58.0	2211-2217 bp	1.0	63.6