Hotspot

ndhC-trnV-UAC

LSC · 52629-54382 · 1754 bp

Back to Sonneratia

Pi 0.00179
MarkerSeek score 51.60
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1754
variable_sites
8
pi
0.00179
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCGGAAAT ACATCACAAACACAAGGGCT 50.0 45.0 57.9 57.9 1904-1908 bp 1.0 78.6
ndhC-trnV-UAC_p2 AATTGGGGCTAAAACTCCGG ACATCACAAACACAAGGGCT 50.0 45.0 58.2 57.9 1908-1912 bp 1.0 78.5
ndhC-trnV-UAC_p3 GGGGCTAAAACTCCGGAAAT CACATCACAAACACAAGGGC 50.0 50.0 57.9 57.9 1905-1909 bp 1.0 78.1
ndhC-trnV-UAC_p4 AATTGGGGCTAAAACTCCGG CACATCACAAACACAAGGGC 50.0 50.0 58.2 57.9 1909-1913 bp 1.0 78.0
ndhC-trnV-UAC_p5 GGGGCTAAAACTCCGGAAAT AGGGCTATAGCTCAGTTGGT 50.0 50.0 57.9 57.8 1890-1894 bp 1.0 77.8