Hotspot

psaA-ycf3

LSC · 43194-43886 · 693 bp

Back to Sonneratia

Pi 0.00275

MarkerSeek score 53.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 693
variable_sites: 6
pi: 0.00275
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GGGGCCCTTAGCTATTGTTC	ATCAAGCCGCTGAGTATTGG	55.0	50.0	58.0	58.0	910-914 bp	1.0	80.1
psaA-ycf3_p2	TGAGAAATGACCCGGTTTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	889-893 bp	1.0	79.5
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	870-874 bp	1.0	79.4
psaA-ycf3_p4	GGGGCCCTTAGCTATTGTTC	CGATCAAGCCGCTGAGTATT	55.0	50.0	58.0	58.1	912-916 bp	1.0	79.3
psaA-ycf3_p5	TGAGAAATGACCCGGTTTGG	CGATCAAGCCGCTGAGTATT	50.0	50.0	58.1	58.1	891-895 bp	1.0	78.7