Hotspot

lhbA-trnG-UCC

LSC · 37465-37804 · 340 bp

Back to Sonneratia

Pi 0.00968

MarkerSeek score 40.40

Primer available yes

Label lhbA-trnG-UCC

Feature Metadata

label_name: lhbA-trnG-UCC
feature_type: igs
region: LSC
length_bp: 340
variable_sites: 9
pi: 0.00968
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 40.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
lhbA-trnG-UCC_p1	CTCCTGAAGGTTGGTCAAGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.1	57.8	487-491 bp	1.0	73.3
lhbA-trnG-UCC_p2	AGTCTTTCTGGTGGGTATCCT	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	57.8	57.8	423-427 bp	1.0	71.4
lhbA-trnG-UCC_p3	CCCGTTGTATTTGCTTCTCC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.8	57.8	503-507 bp	1.0	71.1
lhbA-trnG-UCC_p4	CTTCTCCTGAAGGTTGGTCA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.8	57.8	490-494 bp	1.0	70.9
lhbA-trnG-UCC_p5	TCTCCTGAAGGTTGGTCAAG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.8	57.8	488-492 bp	1.0	70.9