Hotspot

trnE-UUC-trnM-CAU

LSC · 31911-32891 · 981 bp

Back to Sonneratia

Pi 0.00427

MarkerSeek score 54.10

Primer available yes

Label trnE-UUC-trnM-CAU

Feature Metadata

label_name: trnE-UUC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 981
variable_sites: 12
pi: 0.00427
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnM-CAU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	1113-1152 bp	1.0	79.8
trnE-UUC-trnM-CAU_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	1092-1131 bp	1.0	79.3
trnE-UUC-trnM-CAU_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	1089-1128 bp	1.0	79.1
trnE-UUC-trnM-CAU_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	1075-1114 bp	1.0	79.0
trnE-UUC-trnM-CAU_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	1054-1093 bp	1.0	78.8