Hotspot

psbM-trnD-GUC

LSC · 30282-31191 · 910 bp

Back to Sonneratia

Pi 0.00306

MarkerSeek score 50.20

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 910
variable_sites: 7
pi: 0.00306
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGGCAGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	42.9	50.0	55.5	58.5	1026-1037 bp	1.0	49.4
psbM-trnD-GUC_p2	AAGGCAGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	40.9	50.0	56.3	58.5	1027-1038 bp	1.0	47.6
psbM-trnD-GUC_p3	AGGCAGTAGGAACTAGAATGAA	TTCAATTGGTCAGAGCACCG	40.9	50.0	56.3	58.5	1026-1037 bp	1.0	47.6
psbM-trnD-GUC_p4	TCAATCACTTTGACTGACTGT	TTCAATTGGTCAGAGCACCG	38.1	50.0	55.1	58.5	1070-1081 bp	1.0	46.2
psbM-trnD-GUC_p5	GGAACTAGAATGAAAAGTGCAGT	TTCAATTGGTCAGAGCACCG	39.1	50.0	57.1	58.5	1018-1029 bp	1.0	45.8