Hotspot

atpH-atpI

LSC · 13930-14818 · 889 bp

Back to Sonneratia

Pi 0.00504

MarkerSeek score 43.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 889
variable_sites: 13
pi: 0.00504
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	ATTTTTGCAACCTTAGCCGC	45.0	45.0	58.0	57.9	974-981 bp	1.0	80.5
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	942-949 bp	1.0	79.7
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	ATTTTTGCAACCTTAGCCGC	45.0	45.0	58.2	57.9	975-982 bp	1.0	79.3
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.2	57.8	943-950 bp	1.0	78.6
atpH-atpI_p5	TAACGGAAGCGGCAGAAATC	ATTTTTGCAACCTTAGCCGC	50.0	45.0	58.6	57.9	973-980 bp	1.0	75.7