Hotspot

rps16-trnQ-UUG

LSC · 6249-6823 · 575 bp

Back to Sonneratia

Pi 0.00605

MarkerSeek score 46.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 575
variable_sites: 10
pi: 0.00605
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TATGTCTGTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	653-658 bp	1.0	81.0
rps16-trnQ-UUG_p2	GCACGTTGCTTTTTACCACA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.8	58.1	638-643 bp	1.0	79.7
rps16-trnQ-UUG_p3	AGTCGCACGTTGCTTTTTAC	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.6	58.1	642-647 bp	1.0	78.2
rps16-trnQ-UUG_p4	CAAGTCGCACGTTGCTTTTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.5	58.1	644-649 bp	1.0	77.6
rps16-trnQ-UUG_p5	TATGTCTGTCAAGTCGCACG	GGGTTTTGGTCCCGGTATTC	50.0	55.0	58.0	58.5	674-679 bp	1.0	77.3