Hotspot

trnH-GUG-psbA

LSC · 91-404 · 314 bp

Back to Sonneratia

Pi 0.0126

MarkerSeek score 55.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 314
variable_sites: 11
pi: 0.0126
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	429-440 bp	1.0	81.1
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	58.1	57.9	379-390 bp	1.0	81.1
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	443-454 bp	1.0	81.0
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	442-453 bp	1.0	81.0
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	444-455 bp	1.0	81.0