Hotspot

ycf1

SSC · 124875-130343 · 5469 bp

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Pi 0.0267

MarkerSeek score 69.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5469
variable_sites: 491
pi: 0.0267
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGAATGGGAATGGTAAGGGATG	CCTTTTGGTTCTCCCCGAAA	45.5	50.0	57.8	58.0	176-230 bp	1.0	74.5
ycf1_p2	TGAATGGGAATGGTAAGGGA	CCTTTTGGTTCTCCCCGAAA	45.0	50.0	55.8	58.0	176-230 bp	1.0	74.1
ycf1_p3	TGAATGGGAATGGTAAGGGATG	AATCCGATTTCCGCAGAGAC	45.5	50.0	57.8	58.1	859-913 bp	0.9	70.1
ycf1_p4	TGAATGGGAATGGTAAGGGATG	CAATCCGATTTCCGCAGAGA	45.5	50.0	57.8	58.1	860-914 bp	0.9	70.0
ycf1_p5	TGAATGGGAATGGTAAGGGATG	CCTTCCTTTTGGTTCTCCCC	45.5	55.0	57.8	58.1	180-234 bp	0.9	69.9