Hotspot

rps15-ycf1

SSC · 124540-124874 · 335 bp

Back to Sonerila

Pi 0.0602

MarkerSeek score 79.80

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 335
variable_sites: 61
pi: 0.0602
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TGCATTGATGGGTGATGAGG	TGGAAGCCGTTTTAGTCTGT	50.0	45.0	57.9	57.1	803-871 bp	1.0	84.1
rps15-ycf1_p2	GCATTGATGGGTGATGAGGT	TGGAAGCCGTTTTAGTCTGT	50.0	45.0	57.9	57.1	802-870 bp	1.0	84.1
rps15-ycf1_p3	CCGCTGCGAGAAATAGTCTT	TGGAAGCCGTTTTAGTCTGT	50.0	45.0	58.1	57.1	528-560 bp	1.0	83.8
rps15-ycf1_p4	TTTTCCATCCCTCTGTGCAG	TGGAAGCCGTTTTAGTCTGT	50.0	45.0	57.8	57.1	893-961 bp	1.0	83.0
rps15-ycf1_p5	TAACTGTTCGGTTTTCGGCA	TGGAAGCCGTTTTAGTCTGT	45.0	45.0	58.0	57.1	916-984 bp	0.9	78.6