Hotspot

ndhG-ndhI

SSC · 119839-120237 · 399 bp

Back to Sonerila

Pi 0.0458

MarkerSeek score 79.30

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 399
variable_sites: 53
pi: 0.0458
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CCCATTCCCAACAGTCCAAA	TTTGTGGCAATTGCGTTGAG	50.0	45.0	58.0	58.1	882-894 bp	1.0	89.2
ndhG-ndhI_p2	TCCGTACCAAGACGTATCGA	TTTGTGGCAATTGCGTTGAG	50.0	45.0	58.0	58.1	954-966 bp	0.9	84.5
ndhG-ndhI_p3	CCCATTCCCAACAGTCCAAA	ATTGGAAATTGGAAGCCGGT	50.0	45.0	58.0	58.1	954-966 bp	0.9	83.4
ndhG-ndhI_p4	TTGACGGGCAACAACAATTG	ATTGGAAATTGGAAGCCGGT	45.0	45.0	57.8	58.1	1188-1200 bp	0.9	80.7
ndhG-ndhI_p5	TCCGTACCAAGACGTATCGA	ATTGGAAATTGGAAGCCGGT	50.0	45.0	58.0	58.1	1026-1038 bp	0.8	78.9