Hotspot

ndhC-trnV-UAC

LSC · 51263-51917 · 655 bp

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Pi 0.0389
MarkerSeek score 83.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
655
variable_sites
82
pi
0.0389
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.97
markerseek_score
83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCCGGAAATTAGAAATGCCA GGTTCGAATCCGTATAGCCC 40.0 55.0 55.0 57.9 543-841 bp 1.0 67.5
ndhC-trnV-UAC_p2 TCCGGAAATTAGAAATGCCAA GGTTCGAATCCGTATAGCCC 38.1 55.0 55.8 57.9 543-841 bp 1.0 66.1
ndhC-trnV-UAC_p3 TTCCGGAAATTAGAAATGCCA GGTTCGAATCCGTATAGCCC 38.1 55.0 55.8 57.9 544-842 bp 1.0 66.1
ndhC-trnV-UAC_p4 AATGCCAAAAGTGGAATACCA GGTTCGAATCCGTATAGCCC 38.1 55.0 56.0 57.9 530-828 bp 0.9 63.5
ndhC-trnV-UAC_p5 GAAATGCCAAAAGTGGAATACCA GGTTCGAATCCGTATAGCCC 39.1 55.0 57.9 57.9 532-830 bp 0.9 62.2