Hotspot

trnS-GCU-trnR-UCU

LSC · 7971-9746 · 1776 bp

Back to Sonerila

Pi 0.0346

MarkerSeek score 84.60

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1776
variable_sites: 197
pi: 0.0346
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	CAATCCGACGCTTTAGTCCA	AGAACTTGCGTCCAATAGGA	50.0	45.0	58.0	56.6	1880-1960 bp	1.0	79.7
trnS-GCU-trnR-UCU_p2	AATCCGACGCTTTAGTCCAC	AGAACTTGCGTCCAATAGGA	50.0	45.0	58.0	56.6	1879-1959 bp	1.0	79.7
trnS-GCU-trnR-UCU_p3	CAATCCGACGCTTTAGTCCA	AAGAACTTGCGTCCAATAGGA	50.0	42.9	58.0	57.3	1881-1961 bp	1.0	77.7
trnS-GCU-trnR-UCU_p4	CAATCCGACGCTTTAGTCCA	ACATAGAAGAACTTGCGTCCA	50.0	42.9	58.0	57.3	1891-1967 bp	0.9	71.9
trnS-GCU-trnR-UCU_p5	AATCCGACGCTTTAGTCCAC	ACATAGAAGAACTTGCGTCCA	50.0	42.9	58.0	57.3	1890-1966 bp	0.9	71.9