Hotspot

trnQ-UUG-psbK

LSC · 6809-7136 · 328 bp

Back to Sonerila

Pi 0.0415

MarkerSeek score 79.30

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 328
variable_sites: 46
pi: 0.0415
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	GACCAAAACCCGTTGCCTTA	AGGGTGAAATGAAAACAGAAGT	50.0	36.4	58.7	56.1	385-417 bp	1.0	55.3
trnQ-UUG-psbK_p2	GAATAGCGGGACCAAAACCC	AGGGTGAAATGAAAACAGAAGT	55.0	36.4	58.9	56.1	394-426 bp	1.0	53.6
trnQ-UUG-psbK_p3	AAAACCCGTTGCCTTACCAC	AGGGTGAAATGAAAACAGAAGT	50.0	36.4	59.0	56.1	381-413 bp	1.0	53.0
trnQ-UUG-psbK_p4	ACCAAAACCCGTTGCCTTAC	AGGGTGAAATGAAAACAGAAGT	50.0	36.4	59.0	56.1	384-416 bp	1.0	53.0
trnQ-UUG-psbK_p5	AAAAGGTATTCCCTGGGACG	AGGGTGAAATGAAAACAGAAGT	50.0	36.4	57.2	56.1	458-463 bp	0.8	40.6