Hotspot

trnK-UUU-trnQ-UUG

LSC · 4247-6736 · 2490 bp

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Pi 0.0313

MarkerSeek score 75.90

Primer available yes

Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name: trnK-UUU-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 2490
variable_sites: 275
pi: 0.0313
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	AAGCCGAGTACTCTACCGTT	TTCGGAGGTTCGAATCCTTC	50.0	50.0	58.2	57.3	2389-2554 bp	1.0	85.3
trnK-UUU-trnQ-UUG_p2	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2386-2551 bp	0.9	85.0
trnK-UUU-trnQ-UUG_p3	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2385-2550 bp	0.9	85.0
trnK-UUU-trnQ-UUG_p4	CCGCACTTAAAAGCCGAGTA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.3	58.1	2395-2560 bp	0.9	84.1
trnK-UUU-trnQ-UUG_p5	TACCGTTGAGTTAGCAACCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	2372-2537 bp	0.9	82.7