Hotspot

rpl32-ndhF

Genome · 123784-124865 · 1082 bp

Back to Sonchus

Pi 0.0106

MarkerSeek score 66.50

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: Genome
length_bp: 1082
variable_sites: 41
pi: 0.0106
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CCGCCCAATATCCTTTCCTT	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	57.6	58.0	1140-1270 bp	1.0	83.5
rpl32-ndhF_p2	CCGCCCAATATCCTTTCCTT	TGCCGTCGGAAAAAGAAGAA	50.0	45.0	57.6	58.0	1138-1268 bp	1.0	83.4
rpl32-ndhF_p3	CCGCCCAATATCCTTTCCTT	TTGCCGTCGGAAAAAGAAGA	50.0	45.0	57.6	58.0	1139-1269 bp	1.0	83.4
rpl32-ndhF_p4	CCGCCCAATATCCTTTCCTT	TAGGAACTGGGAGTGGAAGG	50.0	55.0	57.6	58.0	1100-1230 bp	1.0	83.4
rpl32-ndhF_p5	TGACAAAGCTTTTAACGCCG	GTTGCCGTCGGAAAAAGAAG	45.0	50.0	57.6	58.0	1157-1287 bp	1.0	83.2