Hotspot

HRV17_pgt015-HRV17_pgt016

Genome · 46474-47054 · 581 bp

Back to Sonchus

Pi 0.0124

MarkerSeek score 64.80

Primer available yes

Label HRV17_pgt015-HRV17_pgt016

Feature Metadata

label_name: HRV17_pgt015-HRV17_pgt016
feature_type: igs
region: Genome
length_bp: 581
variable_sites: 27
pi: 0.0124
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
HRV17_pgt015-HRV17_pgt016_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	592-666 bp	1.0	84.3
HRV17_pgt015-HRV17_pgt016_p2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	624-698 bp	1.0	83.3
HRV17_pgt015-HRV17_pgt016_p3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	586-660 bp	1.0	83.0
HRV17_pgt015-HRV17_pgt016_p4	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	50.0	50.0	57.8	57.6	618-692 bp	1.0	82.0
HRV17_pgt015-HRV17_pgt016_p5	GCGATGCTCTAACCTCTGAG	CGTAGCGTCTACCGATTTCG	55.0	55.0	57.9	58.6	591-665 bp	1.0	81.4