Hotspot

atpA-HRV17_pgt009

Genome · 29726-29856 · 131 bp

Back to Sonchus

Pi 0.0257

MarkerSeek score 66.20

Primer available yes

Label atpA-HRV17_pgt009

Feature Metadata

label_name: atpA-HRV17_pgt009
feature_type: igs
region: Genome
length_bp: 131
variable_sites: 11
pi: 0.0257
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-HRV17_pgt009_p1	TACTTCAGGAACAAGCAGCC	GCAATGAAAAGCGTCCATTGT	50.0	42.9	57.8	58.3	200-240 bp	1.0	75.8
atpA-HRV17_pgt009_p2	CATTCACCGAGGAAGCAGAA	GCAATGAAAAGCGTCCATTGT	50.0	42.9	57.9	58.3	263-303 bp	1.0	75.6
atpA-HRV17_pgt009_p3	AAGACATTCACCGAGGAAGC	GCAATGAAAAGCGTCCATTGT	50.0	42.9	57.9	58.3	267-307 bp	1.0	75.6
atpA-HRV17_pgt009_p4	CATTCACCGAGGAAGCAGAA	GCAATGAAAAGCGTCCATTG	50.0	45.0	57.9	56.6	263-303 bp	1.0	74.8
atpA-HRV17_pgt009_p5	AAGACATTCACCGAGGAAGC	GCAATGAAAAGCGTCCATTG	50.0	45.0	57.9	56.6	267-307 bp	1.0	74.8