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HRV17_pgt005-petN

Genome · 9487-10158 · 672 bp

Back to Sonchus

Pi 0.00682

MarkerSeek score 57.10

Primer available yes

Label HRV17_pgt005-petN

Feature Metadata

label_name: HRV17_pgt005-petN
feature_type: igs
region: Genome
length_bp: 672
variable_sites: 20
pi: 0.00682
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
HRV17_pgt005-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	729-885 bp	1.0	83.0
HRV17_pgt005-petN_p2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	747-903 bp	1.0	80.7
HRV17_pgt005-petN_p3	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.1	57.1	728-884 bp	1.0	77.0
HRV17_pgt005-petN_p4	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	50.0	50.0	58.1	58.9	737-893 bp	1.0	76.9
HRV17_pgt005-petN_p5	ATTGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.1	58.2	786-942 bp	1.0	76.2