Hotspot

HRV17_pgt001-psbA

Genome · 78-478 · 401 bp

Back to Sonchus

Pi 0.016

MarkerSeek score 63.00

Primer available yes

Label HRV17_pgt001-psbA

Feature Metadata

label_name: HRV17_pgt001-psbA
feature_type: igs
region: Genome
length_bp: 401
variable_sites: 33
pi: 0.016
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
HRV17_pgt001-psbA_p1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	55.0	55.0	58.1	58.3	519-550 bp	1.0	81.9
HRV17_pgt001-psbA_p2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	533-564 bp	1.0	81.7
HRV17_pgt001-psbA_p3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	532-563 bp	1.0	81.7
HRV17_pgt001-psbA_p4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	534-565 bp	1.0	81.7
HRV17_pgt001-psbA_p5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	55.0	50.0	58.1	57.6	520-551 bp	1.0	81.4