primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
HRV17_pgt001-psbA_p1	HRV17_pgt001-psbA	1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	78	478	519-550	1.000000	81.9
HRV17_pgt001-psbA_p2	HRV17_pgt001-psbA	2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.469587	78	478	533-564	1.000000	81.7
HRV17_pgt001-psbA_p3	HRV17_pgt001-psbA	3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	78	478	532-563	1.000000	81.7
HRV17_pgt001-psbA_p4	HRV17_pgt001-psbA	4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	78	478	534-565	1.000000	81.7
HRV17_pgt001-psbA_p5	HRV17_pgt001-psbA	5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	20	20	55.0	50.0	58.1	57.6	0.0	0.0	0.525510	78	478	520-551	1.000000	81.4
HRV17_pgt005-petN_p1	HRV17_pgt005-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	9487	10158	729-885	1.000000	83.0
HRV17_pgt005-petN_p2	HRV17_pgt005-petN	2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	9487	10158	747-903	1.000000	80.7
HRV17_pgt005-petN_p3	HRV17_pgt005-petN	3	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	20	20	50.0	50.0	58.1	57.1	0.0	0.0	0.974912	9487	10158	728-884	1.000000	77.0
HRV17_pgt005-petN_p4	HRV17_pgt005-petN	4	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	20	20	50.0	50.0	58.1	58.9	0.0	0.0	0.984078	9487	10158	737-893	1.000000	76.9
HRV17_pgt005-petN_p5	HRV17_pgt005-petN	5	ATTGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	20	20	45.0	50.0	57.1	58.2	0.0	0.0	1.063167	9487	10158	786-942	1.000000	76.2
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTT	20	20	50.0	40.0	58.2	55.6	0.0	0.0	2.573941	10249	10731	641-649	1.000000	63.3
petN-psbM_p2	petN-psbM	2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTTT	20	21	50.0	38.1	58.2	56.4	0.0	0.0	2.785752	10249	10731	641-649	1.000000	61.6
petN-psbM_p3	petN-psbM	3	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTT	20	20	50.0	40.0	57.1	55.6	0.0	0.0	3.315377	10249	10731	642-650	1.000000	57.3
petN-psbM_p4	petN-psbM	4	CTCGCTTGGGCTGCTTTAAT	TGCTACTGCACTGTTCATTT	20	20	50.0	40.0	58.9	55.6	0.0	0.0	3.324543	10249	10731	633-641	1.000000	57.3
petN-psbM_p5	petN-psbM	5	TGGGCTGCTTTAATGGTAGTC	TGCTACTGCACTGTTCATTT	21	20	47.6	40.0	58.0	55.6	0.0	0.0	3.423261	10249	10731	627-635	1.000000	56.6
HRV17_pgt008-rpoB_p1	HRV17_pgt008-rpoB	1	GGGACTTGAACCCTCGTTTT	AGTTCTTCTGTCAAACCCTGA	20	21	50.0	42.9	58.0	56.8	0.0	0.0	2.237066	12000	12914	1034-1079	1.000000	67.8
HRV17_pgt008-rpoB_p2	HRV17_pgt008-rpoB	2	TAACCACTGGACCATAGGGG	AGTTCTTCTGTCAAACCCTGA	20	21	55.0	42.9	58.1	56.8	14.8	0.0	2.326325	12000	12914	993-1038	1.000000	67.4
HRV17_pgt008-rpoB_p3	HRV17_pgt008-rpoB	3	CCCTCGTTTTCTCCGTGAAA	AGTTCTTCTGTCAAACCCTGA	20	21	50.0	42.9	58.1	56.8	0.0	0.0	2.340425	12000	12914	1024-1069	1.000000	67.1
HRV17_pgt008-rpoB_p4	HRV17_pgt008-rpoB	4	TTGAACCCTCGTTTTCTCCG	AGTTCTTCTGTCAAACCCTGA	20	21	50.0	42.9	58.1	56.8	0.0	0.0	2.340425	12000	12914	1029-1074	1.000000	67.0
HRV17_pgt008-rpoB_p5	HRV17_pgt008-rpoB	5	GGGACTTGAACCCTCGTTTT	TCAAACCCTGATCAATGAACCT	20	22	50.0	40.9	58.0	57.6	0.0	0.0	2.409970	12000	12914	1024-1069	1.000000	66.5
atpA-HRV17_pgt009_p1	atpA-HRV17_pgt009	1	TACTTCAGGAACAAGCAGCC	GCAATGAAAAGCGTCCATTGT	20	21	50.0	42.9	57.8	58.3	0.0	20.2	1.508929	29726	29856	200-240	1.000000	75.8
atpA-HRV17_pgt009_p2	atpA-HRV17_pgt009	2	CATTCACCGAGGAAGCAGAA	GCAATGAAAAGCGTCCATTGT	20	21	50.0	42.9	57.9	58.3	0.0	20.2	1.423066	29726	29856	263-303	1.000000	75.6
atpA-HRV17_pgt009_p3	atpA-HRV17_pgt009	3	AAGACATTCACCGAGGAAGC	GCAATGAAAAGCGTCCATTGT	20	21	50.0	42.9	57.9	58.3	0.0	20.2	1.424529	29726	29856	267-307	1.000000	75.6
atpA-HRV17_pgt009_p4	atpA-HRV17_pgt009	4	CATTCACCGAGGAAGCAGAA	GCAATGAAAAGCGTCCATTG	20	20	50.0	45.0	57.9	56.6	0.0	17.2	1.525779	29726	29856	263-303	1.000000	74.8
atpA-HRV17_pgt009_p5	atpA-HRV17_pgt009	5	AAGACATTCACCGAGGAAGC	GCAATGAAAAGCGTCCATTG	20	20	50.0	45.0	57.9	56.6	0.0	17.2	1.527243	29726	29856	267-307	1.000000	74.8
ycf3-HRV17_pgt014_p1	ycf3-HRV17_pgt014	1	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	21	20	47.6	50.0	58.0	58.2	8.2	0.0	1.191833	44113	45032	967-1036	1.000000	76.6
ycf3-HRV17_pgt014_p2	ycf3-HRV17_pgt014	2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	21	20	47.6	45.0	58.0	57.8	8.2	0.0	1.233148	44113	45032	917-986	1.000000	76.3
ycf3-HRV17_pgt014_p3	ycf3-HRV17_pgt014	3	CATTTATCCGCGATCTAGGCA	GGATTCGAACCCTCGGTAAG	21	20	47.6	55.0	58.0	57.8	8.2	0.0	1.260963	44113	45032	955-1024	1.000000	76.0
ycf3-HRV17_pgt014_p4	ycf3-HRV17_pgt014	4	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	21	20	47.6	50.0	58.0	58.3	8.2	0.0	1.301418	44113	45032	919-988	1.000000	75.8
ycf3-HRV17_pgt014_p5	ycf3-HRV17_pgt014	5	CATTTATCCGCGATCTAGGCA	CCTCGGTAAGCAAAAGCCTA	21	20	47.6	50.0	58.0	57.6	8.2	0.0	1.437074	44113	45032	945-1014	1.000000	74.7
HRV17_pgt015-HRV17_pgt016_p1	HRV17_pgt015-HRV17_pgt016	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	46474	47054	592-666	1.000000	84.3
HRV17_pgt015-HRV17_pgt016_p2	HRV17_pgt015-HRV17_pgt016	2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	46474	47054	624-698	1.000000	83.3
HRV17_pgt015-HRV17_pgt016_p3	HRV17_pgt015-HRV17_pgt016	3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	46474	47054	586-660	1.000000	83.0
HRV17_pgt015-HRV17_pgt016_p4	HRV17_pgt015-HRV17_pgt016	4	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	20	20	50.0	50.0	57.8	57.6	15.5	0.0	0.651279	46474	47054	618-692	1.000000	82.0
HRV17_pgt015-HRV17_pgt016_p5	HRV17_pgt015-HRV17_pgt016	5	GCGATGCTCTAACCTCTGAG	CGTAGCGTCTACCGATTTCG	20	20	55.0	55.0	57.9	58.6	0.0	10.2	0.749620	46474	47054	591-665	1.000000	81.4
HRV17_pgt017-ndhJ_p1	HRV17_pgt017-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.273485	47988	48577	507-734	1.000000	75.9
HRV17_pgt017-ndhJ_p2	HRV17_pgt017-ndhJ	2	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.275704	47988	48577	506-733	1.000000	75.9
HRV17_pgt017-ndhJ_p3	HRV17_pgt017-ndhJ	3	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGATGGCC	20	21	50.0	47.6	58.1	57.7	0.0	0.0	1.349030	47988	48577	514-741	1.000000	75.3
HRV17_pgt017-ndhJ_p4	HRV17_pgt017-ndhJ	4	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTACG	20	21	50.0	47.6	58.1	57.5	0.0	3.3	1.537359	47988	48577	508-735	1.000000	73.8
HRV17_pgt017-ndhJ_p5	HRV17_pgt017-ndhJ	5	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACGT	20	21	50.0	47.6	58.1	58.6	0.0	3.3	1.647337	47988	48577	507-734	1.000000	72.9
ndhC-HRV17_pgt018_p1	ndhC-HRV17_pgt018	1	TCCTTTGCTACTCGGAGCTA	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.9	58.1	4.6	0.0	0.246856	50250	51464	1334-1396	1.000000	83.9
ndhC-HRV17_pgt018_p2	ndhC-HRV17_pgt018	2	ACTCGGAGCTAAAAATCCCG	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.7	58.1	4.6	0.0	0.418656	50250	51464	1325-1387	1.000000	82.6
ndhC-HRV17_pgt018_p3	ndhC-HRV17_pgt018	3	TTCTCCGGTCCTTTGCTACT	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	58.4	58.1	0.0	0.0	0.466616	50250	51464	1342-1404	1.000000	82.1
ndhC-HRV17_pgt018_p4	ndhC-HRV17_pgt018	4	TTTCTCCGGTCCTTTGCTAC	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.5	58.1	0.0	0.0	0.575098	50250	51464	1343-1405	1.000000	81.3
ndhC-HRV17_pgt018_p5	ndhC-HRV17_pgt018	5	CTCGGAGCTAAAAATCCCGA	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.4	58.1	9.7	0.0	0.704436	50250	51464	1324-1386	1.000000	80.3
petA-psbJ_p1	petA-psbJ	1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	58.0	57.6	17.6	4.1	0.411974	63093	63867	897-919	1.000000	81.7
petA-psbJ_p2	petA-psbJ	2	ACGTGTACAAGGCCTTTTGT	TGTGATCGGTTTAGTAGGCG	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.619192	63093	63867	935-957	1.000000	79.9
petA-psbJ_p3	petA-psbJ	3	ACGTGTACAAGGCCTTTTGT	GTGATCGGTTTAGTAGGCGT	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.620943	63093	63867	934-956	1.000000	79.8
petA-psbJ_p4	petA-psbJ	4	TCCATTACGTGTACAAGGCC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.6	57.6	13.5	4.1	0.754934	63093	63867	903-925	1.000000	78.9
petA-psbJ_p5	petA-psbJ	5	CCATTACGTGTACAAGGCCT	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.6	57.6	13.5	4.1	0.761562	63093	63867	902-924	1.000000	78.9
HRV17_pgp043_p1	HRV17_pgp043	1	TTGTTCGATGAGCAGATCCA	TCTGGATCCAAAGAATCAGTCA	20	22	45.0	40.9	56.9	56.8	0.0	17.0	4.241922	82323	82442	298-351	1.000000	53.9
HRV17_pgp043_p2	HRV17_pgp043	2	TGTTCGATGAGCAGATCCAT	TCTGGATCCAAAGAATCAGTCA	20	22	45.0	40.9	56.7	56.8	0.0	17.0	4.471144	82323	82442	297-350	1.000000	52.1
HRV17_pgp043_p3	HRV17_pgp043	3	TTGTTCGATGAGCAGATCCA	TGGATCCAAAGAATCAGTCAAGA	20	23	45.0	39.1	56.9	57.5	0.0	13.5	4.560823	82323	82442	296-349	1.000000	51.4
HRV17_pgp043_p4	HRV17_pgp043	4	TTGTTCGATGAGCAGATCCA	TCTGGATCCAAAGAATCAGTCAA	20	23	45.0	39.1	56.9	57.5	0.0	17.0	4.560823	82323	82442	298-351	1.000000	51.3
HRV17_pgp043_p5	HRV17_pgp043	5	TTGTTCGATGAGCAGATCCA	AACCAACTCATCACTTCGTA	20	20	45.0	40.0	56.9	54.3	0.0	0.0	4.718473	82323	82442	321-374	1.000000	49.8
ndhD-ccsA_p1	ndhD-ccsA	1	TCGATCGCCATTTTTCTACCT	TGGCGACTCTAGGCTTTCTT	21	20	42.9	50.0	57.5	58.7	11.1	0.0	2.269834	121653	121915	471-492	1.000000	67.2
ndhD-ccsA_p2	ndhD-ccsA	2	CGATCGCCATTTTTCTACCTG	TGGCGACTCTAGGCTTTCTT	21	20	47.6	50.0	57.4	58.7	0.3	0.0	2.325260	121653	121915	470-491	1.000000	66.8
ndhD-ccsA_p3	ndhD-ccsA	3	TCAGTTGACAAGGTTGAAGGT	TGGCGACTCTAGGCTTTCTT	21	20	42.9	50.0	57.4	58.7	0.0	0.0	2.377845	121653	121915	408-429	1.000000	66.7
ndhD-ccsA_p4	ndhD-ccsA	4	TCGATCGCCATTTTTCTACCT	CAATTGTGGCGACTCTAGGC	21	20	42.9	55.0	57.5	59.0	11.1	0.0	2.527127	121653	121915	477-498	1.000000	65.1
ndhD-ccsA_p5	ndhD-ccsA	5	TCGATCGCCATTTTTCTACCT	GCAATTGTGGCGACTCTAGG	21	20	42.9	55.0	57.5	59.0	11.1	7.4	2.527127	121653	121915	478-499	0.941176	62.2
rpl32-ndhF_p1	rpl32-ndhF	1	CCGCCCAATATCCTTTCCTT	GTTGCCGTCGGAAAAAGAAG	20	20	50.0	50.0	57.6	58.0	0.0	17.5	0.397386	123784	124865	1140-1270	1.000000	83.5
rpl32-ndhF_p2	rpl32-ndhF	2	CCGCCCAATATCCTTTCCTT	TGCCGTCGGAAAAAGAAGAA	20	20	50.0	45.0	57.6	58.0	0.0	17.5	0.408676	123784	124865	1138-1268	1.000000	83.4
rpl32-ndhF_p3	rpl32-ndhF	3	CCGCCCAATATCCTTTCCTT	TTGCCGTCGGAAAAAGAAGA	20	20	50.0	45.0	57.6	58.0	0.0	17.5	0.408676	123784	124865	1139-1269	1.000000	83.4
rpl32-ndhF_p4	rpl32-ndhF	4	CCGCCCAATATCCTTTCCTT	TAGGAACTGGGAGTGGAAGG	20	20	50.0	55.0	57.6	58.0	0.0	0.0	0.412351	123784	124865	1100-1230	1.000000	83.4
rpl32-ndhF_p5	rpl32-ndhF	5	TGACAAAGCTTTTAACGCCG	GTTGCCGTCGGAAAAAGAAG	20	20	45.0	50.0	57.6	58.0	9.5	17.5	0.425347	123784	124865	1157-1287	1.000000	83.2