Hotspot

rpl20-rps12

LSC · 68638-69403 · 766 bp

Back to Solms-laubachia

Pi 0.0134

MarkerSeek score 83.30

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 766
variable_sites: 77
pi: 0.0134
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTATATATCCGCGT	ACAGCCAATCCGAAATGTCA	50.0	45.0	58.0	57.8	872-889 bp	1.0	89.4
rpl20-rps12_p2	GAAGTTTTGTTCTGCGCCTC	ACAGCCAATCCGAAATGTCA	50.0	45.0	58.0	57.8	890-907 bp	1.0	89.2
rpl20-rps12_p3	TCCGAGCTATATATCCGCGT	GAGGAACATGTACTCGGGTG	50.0	55.0	58.0	57.7	816-833 bp	1.0	88.9
rpl20-rps12_p4	TCCGAGCTATATATCCGCGT	ACAAGACAGCCAATCCGAAA	50.0	45.0	58.0	57.7	877-894 bp	1.0	88.8
rpl20-rps12_p5	GAAGTTTTGTTCTGCGCCTC	ACAAGACAGCCAATCCGAAA	50.0	45.0	58.0	57.7	895-912 bp	1.0	88.6