Hotspot

trnP-CCA-psaJ

LSC · 66269-66645 · 377 bp

Back to Solms-laubachia

Pi 0.0318

MarkerSeek score 74.80

Primer available yes

Label trnP-CCA-psaJ

Feature Metadata

label_name: trnP-CCA-psaJ
feature_type: igs
region: LSC
length_bp: 377
variable_sites: 60
pi: 0.0318
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-CCA-psaJ_p1	ACCCGTGACATTTTGTACCC	GGCGCTACGGAAAGATATGT	50.0	50.0	58.1	57.8	441-479 bp	1.0	90.0
trnP-CCA-psaJ_p2	CCCGTGACATTTTGTACCCA	GGCGCTACGGAAAGATATGT	50.0	50.0	58.1	57.8	440-478 bp	1.0	90.0
trnP-CCA-psaJ_p3	ACAGGATTTGAACCCGTGAC	GGCGCTACGGAAAGATATGT	50.0	50.0	58.1	57.8	452-490 bp	1.0	89.9
trnP-CCA-psaJ_p4	CAGGATTTGAACCCGTGACA	GGCGCTACGGAAAGATATGT	50.0	50.0	58.1	57.8	451-489 bp	1.0	89.9
trnP-CCA-psaJ_p5	TGACAGGATTTGAACCCGTG	GGCGCTACGGAAAGATATGT	50.0	50.0	58.1	57.8	454-492 bp	1.0	89.9