Hotspot

ycf4-cemA

LSC · 60298-60738 · 441 bp

Back to Solms-laubachia

Pi 0.0397

MarkerSeek score 90.40

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 441
variable_sites: 74
pi: 0.0397
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 90.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	AGGTCAAGGGGCTATTCCTT	GAGAGAGATCAAACAGGGCA	50.0	50.0	58.0	56.9	618-706 bp	1.0	83.4
ycf4-cemA_p2	TTGGCTTACTTCTTGCGTGT	GAGAGAGATCAAACAGGGCA	45.0	50.0	58.0	56.9	542-630 bp	1.0	83.2
ycf4-cemA_p3	AGGTCAAGGGGCTATTCCTT	CAGAGAGAGATCAAACAGGGC	50.0	52.4	58.0	58.1	620-708 bp	1.0	83.1
ycf4-cemA_p4	AGGTCAAGGGGCTATTCCTT	GCAGAGAGAGATCAAACAGGG	50.0	52.4	58.0	58.1	621-709 bp	1.0	83.1
ycf4-cemA_p5	TTGGCTTACTTCTTGCGTGT	CAGAGAGAGATCAAACAGGGC	45.0	52.4	58.0	58.1	544-632 bp	1.0	82.9