Hotspot

rbcL-accD

LSC · 56397-57071 · 675 bp

Back to Solms-laubachia

Pi 0.0306

MarkerSeek score 82.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 675
variable_sites: 119
pi: 0.0306
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTTGTGAAGTGTGGAAGGA	ATCTCCCCTGTGTATTCCGA	50.0	50.0	58.0	57.5	789-835 bp	1.0	88.0
rbcL-accD_p2	GCTTGTGAAGTGTGGAAGGA	TCTCCCCTGTGTATTCCGAT	50.0	50.0	58.0	57.5	788-834 bp	1.0	88.0
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	ATCTCCCCTGTGTATTCCGA	55.0	50.0	57.7	57.5	808-854 bp	1.0	85.9
rbcL-accD_p4	GAGTCCTGAACTAGCTGCTG	TCTCCCCTGTGTATTCCGAT	55.0	50.0	57.7	57.5	807-853 bp	1.0	85.9
rbcL-accD_p5	TGCTTGTGAAGTGTGGAAGG	TCTCCCCTGTGTATTCCGAT	50.0	50.0	58.3	57.5	789-835 bp	1.0	85.7