Hotspot

ndhC-trnV-GUA

LSC · 50306-51211 · 906 bp

Back to Solms-laubachia

Pi 0.0301

MarkerSeek score 78.60

Primer available yes

Label ndhC-trnV-GUA

Feature Metadata

label_name: ndhC-trnV-GUA
feature_type: igs
region: LSC
length_bp: 906
variable_sites: 160
pi: 0.0301
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-GUA_p1	TGCTAAAACAGGAATAGCACTTG	GTTCGAGTCCGTATAGCCCT	39.1	55.0	57.4	58.7	799-999 bp	0.952381	55.6
ndhC-trnV-GUA_p2	TGCTAAAACAGGAATAGCACT	GTTCGAGTCCGTATAGCCCT	38.1	55.0	55.2	58.7	799-999 bp	0.952381	54.3
ndhC-trnV-GUA_p3	TGCTAAAACAGGAATAGCACTTG	TTCGAGTCCGTATAGCCCTA	39.1	50.0	57.4	57.1	798-998 bp	0.952381	53.6
ndhC-trnV-GUA_p4	TGCTAAAACAGGAATAGCACTTG	TCGAACTCTTTTACCGAGCAG	39.1	47.6	57.4	58.1	827-1027 bp	0.952381	52.6
ndhC-trnV-GUA_p5	GCTAAAACAGGAATAGCACTTGA	GTTCGAGTCCGTATAGCCCT	39.1	55.0	57.1	58.7	798-998 bp	0.904762	51.7