Hotspot

psaA-ycf3

LSC · 41686-42401 · 716 bp

Back to Solms-laubachia

Pi 0.018

MarkerSeek score 85.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 716
variable_sites: 76
pi: 0.018
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	GCTATAACGCTTACTCCCGG	50.0	55.0	57.9	57.9	859-985 bp	1.0	90.0
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GGCTATAACGCTTACTCCCG	50.0	55.0	57.9	57.9	860-986 bp	1.0	89.9
psaA-ycf3_p3	AGAAATGACCGGGTTTAGCC	GCTATAACGCTTACTCCCGG	50.0	55.0	57.9	57.9	876-1002 bp	1.0	89.7
psaA-ycf3_p4	AGAAATGACCGGGTTTAGCC	GGCTATAACGCTTACTCCCG	50.0	55.0	57.9	57.9	877-1003 bp	1.0	89.7
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	ATTGGTTGACCATCACGAGG	50.0	50.0	57.9	57.9	819-945 bp	0.952381	87.6