Hotspot

trnT-ACC-psbD

LSC · 31298-32570 · 1273 bp

Back to Solms-laubachia

Pi 0.03

MarkerSeek score 79.20

Primer available yes

Label trnT-ACC-psbD

Feature Metadata

label_name: trnT-ACC-psbD
feature_type: igs
region: LSC
length_bp: 1273
variable_sites: 186
pi: 0.03
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-ACC-psbD_p1	GTGGTAGAGTAACGCCATGG	CAAAAACAAAGCGGTCCCTC	55.0	50.0	58.1	57.9	927-1542 bp	0.952381	88.5
trnT-ACC-psbD_p2	TCAGTGGTAGAGTAACGCCA	CAAAAACAAAGCGGTCCCTC	50.0	50.0	58.1	57.9	930-1545 bp	0.952381	88.3
trnT-ACC-psbD_p3	GGCGTAAGTCATCGGTTCAA	CAAAAACAAAGCGGTCCCTC	50.0	50.0	58.3	57.9	904-1519 bp	0.952381	86.8
trnT-ACC-psbD_p4	TGGCCCTTTTAACTCAGTGG	CAAAAACAAAGCGGTCCCTC	50.0	50.0	57.7	57.9	943-1558 bp	0.904762	84.8
trnT-ACC-psbD_p5	GGCCCTTTTAACTCAGTGGT	CAAAAACAAAGCGGTCCCTC	50.0	50.0	57.7	57.9	942-1557 bp	0.904762	84.8