Hotspot

atpF-atpH

LSC · 12508-12969 · 462 bp

Back to Solms-laubachia

Pi 0.0294

MarkerSeek score 85.60

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 462
variable_sites: 71
pi: 0.0294
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	TGGCCTGGTTGTAGCATTAG	50.0	50.0	57.9	57.6	556-610 bp	1.0	87.7
atpF-atpH_p2	CCAGTGACCCAAGTAAACGA	TGGCCTGGTTGTAGCATTAG	50.0	50.0	57.5	57.6	529-583 bp	1.0	84.4
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	TTTATGGCCTGGTTGTAGCA	50.0	45.0	57.9	57.1	560-614 bp	1.0	83.9
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GGCCTGGTTGTAGCATTAGC	50.0	55.0	57.9	59.0	555-609 bp	1.0	83.3
atpF-atpH_p5	GGCCAGTGACCCAAGTAAAC	TGGCCTGGTTGTAGCATTAG	55.0	50.0	58.8	57.6	531-585 bp	1.0	82.6