Hotspot

trnS-AGC-trnG-GGA

LSC · 7703-8363 · 661 bp

Back to Solms-laubachia

Pi 0.0606

MarkerSeek score 89.20

Primer available yes

Label trnS-AGC-trnG-GGA

Feature Metadata

label_name: trnS-AGC-trnG-GGA
feature_type: igs
region: LSC
length_bp: 661
variable_sites: 177
pi: 0.0606
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 89.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-AGC-trnG-GGA_p1	CCAACGCTTTAGTCCACTCA	TCGGAAGATGAATCAAACCGA	50.0	42.9	57.8	57.4	534-882 bp	1.0	77.9
trnS-AGC-trnG-GGA_p2	AACGCTTTAGTCCACTCAGC	TCGGAAGATGAATCAAACCGA	50.0	42.9	58.2	57.4	532-880 bp	1.0	77.6
trnS-AGC-trnG-GGA_p3	GCTTTAGTCCACTCAGCCAT	TCGGAAGATGAATCAAACCGA	50.0	42.9	57.6	57.4	529-877 bp	1.0	75.9
trnS-AGC-trnG-GGA_p4	TCCAACGCTTTAGTCCACTC	TCGGAAGATGAATCAAACCGA	50.0	42.9	57.5	57.4	535-883 bp	1.0	75.6
trnS-AGC-trnG-GGA_p5	AGTCCACTCAGCCATCTCTC	TCGGAAGATGAATCAAACCGA	55.0	42.9	58.5	57.4	524-872 bp	1.0	75.0