Hotspot

trnH-CAC-psbA

LSC · 77-425 · 349 bp

Back to Solms-laubachia

Pi 0.0821

MarkerSeek score 86.40

Primer available yes

Label trnH-CAC-psbA

Feature Metadata

label_name: trnH-CAC-psbA
feature_type: igs
region: LSC
length_bp: 349
variable_sites: 127
pi: 0.0821
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 86.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-CAC-psbA_p1	CTGCCTTAATCCACTTGGCT	GCTGTTGAGGCTCCATCTAC	50.0	55.0	57.9	58.1	306-456 bp	0.952381	88.6
trnH-CAC-psbA_p2	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	225-474 bp	0.952381	88.4
trnH-CAC-psbA_p3	ACTGCCTTAATCCACTTGGC	GCTGTTGAGGCTCCATCTAC	50.0	55.0	58.2	58.1	307-457 bp	0.952381	88.4
trnH-CAC-psbA_p4	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	222-471 bp	0.904762	86.6
trnH-CAC-psbA_p5	ACTGCCTTAATCCACTTGGC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	58.2	57.9	223-472 bp	0.904762	86.4