Hotspot

ndhA

SSC · 116445-118638 · 2194 bp

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Pi 0.00183

MarkerSeek score 58.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2194
variable_sites: 4
pi: 0.00183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CCGCCGGGGTTTATTAATTTG	TGTTTGAGACAAGTCGTGAA	47.6	40.0	57.9	55.2	2344-2348 bp	1.0	49.3
ndhA_p2	CCGCCGGGGTTTATTAATTTG	TTGTTTGAGACAAGTCGTGA	47.6	40.0	57.9	55.2	2345-2349 bp	1.0	49.3
ndhA_p3	CCGCCGGGGTTTATTAATTTG	TTGTTTGAGACAAGTCGTGAA	47.6	38.1	57.9	56.0	2345-2349 bp	1.0	47.7
ndhA_p4	CCGCCGGGGTTTATTAATTTG	ACCATAGGGAACATATCGTGA	47.6	42.9	57.9	55.9	2401-2405 bp	1.0	47.3
ndhA_p5	CCGCCGGGGTTTATTAATTTG	TGGCCATAATTCATGAACTCAGT	47.6	39.1	57.9	58.1	2425-2429 bp	1.0	46.6