Hotspot

ccsA

SSC · 122835-123803 · 969 bp

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Pi 0.00103

MarkerSeek score 59.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 1
pi: 0.00103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AGAACCGCGTGAATCACTAC	GGGATCCCCACCTTTTTCTT	50.0	50.0	58.0	57.4	1108 bp	1.0	75.0
ccsA_p2	AGAACCGCGTGAATCACTAC	CGGCATGCCATCTTCTAAAC	50.0	50.0	58.0	57.2	1194 bp	1.0	73.9
ccsA_p3	AGAACCGCGTGAATCACTAC	GGCATGCCATCTTCTAAACG	50.0	50.0	58.0	57.2	1193 bp	1.0	73.9
ccsA_p4	GAGAACCGCGTGAATCACTA	GGGATCCCCACCTTTTTCTT	50.0	50.0	57.7	57.4	1109 bp	1.0	72.9
ccsA_p5	GAACCGCGTGAATCACTACA	GGGATCCCCACCTTTTTCTT	50.0	50.0	58.3	57.4	1107 bp	1.0	72.7