Hotspot

rpl32-ndhF

SSC · 124991-125785 · 795 bp

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Pi 0

MarkerSeek score 59.50

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 795
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	ACGACGCCCAATATCCTTTC	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	58.1	58.0	972-978 bp	1.0	79.8
rpl32-ndhF_p2	CGACGCCCAATATCCTTTCA	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	58.1	58.0	971-977 bp	1.0	79.8
rpl32-ndhF_p3	ACGACGCCCAATATCCTTTC	TGCCGTCGGAAAAAGAAGAA	50.0	45.0	58.1	58.0	970-976 bp	1.0	79.7
rpl32-ndhF_p4	ACGACGCCCAATATCCTTTC	TTGCCGTCGGAAAAAGAAGA	50.0	45.0	58.1	58.0	971-977 bp	1.0	79.7
rpl32-ndhF_p5	CGACGCCCAATATCCTTTCA	TGCCGTCGGAAAAAGAAGAA	50.0	45.0	58.1	58.0	969-975 bp	1.0	79.7