Hotspot

rps12-ycf15

IRb · 98923-99978 · 1056 bp

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Pi 0

MarkerSeek score 59.30

Primer available yes

Label rps12-ycf15

Feature Metadata

label_name: rps12-ycf15
feature_type: igs
region: IRb
length_bp: 1056
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-ycf15_p1	TGGCAACTTTACGTAAGGCA	GAAAGGACTCACTAAGCCGG	45.0	55.0	57.7	58.0	1125-1131 bp	1.0	78.3
rps12-ycf15_p2	TGGCAACTTTACGTAAGGCA	CACGGTACAATCCTCTTCCC	45.0	55.0	57.7	57.7	1214-1220 bp	1.0	75.8
rps12-ycf15_p3	ACGTAAGGCAGAGTTTGGTT	GAAAGGACTCACTAAGCCGG	45.0	55.0	57.4	58.0	1115-1121 bp	1.0	75.3
rps12-ycf15_p4	TGGCAACTTTACGTAAGGCA	TCACTAAGCCGGGATCACTA	45.0	50.0	57.7	57.6	1117-1123 bp	1.0	74.8
rps12-ycf15_p5	TGGCAACTTTACGTAAGGCA	CTCGGTCCACAGAGACAAAA	45.0	50.0	57.7	57.5	1177-1183 bp	1.0	74.1