Hotspot

rpl16-rps3

LSC · 82213-83517 · 1305 bp

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Pi 0.00308

MarkerSeek score 61.10

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1305
variable_sites: 4
pi: 0.00308
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	CAAGTGCCTGAAGAGCGTAT	AGAGAAGGTAGGGTTCCACT	50.0	50.0	58.0	57.0	1537-1540 bp	1.0	72.3
rpl16-rps3_p2	CAAGTGCCTGAAGAGCGTAT	GGTAGGGTTCCACTACAAACC	50.0	52.4	58.0	57.9	1531-1534 bp	1.0	72.0
rpl16-rps3_p3	CAAGTGCCTGAAGAGCGTAT	CCACTACAAACCATTCGAGC	50.0	50.0	58.0	56.8	1522-1525 bp	1.0	71.0
rpl16-rps3_p4	CAAGTGCCTGAAGAGCGTAT	CCACTACAAACCATTCGAGCT	50.0	47.6	58.0	58.3	1522-1525 bp	1.0	70.1
rpl16-rps3_p5	CAAGTGCCTGAAGAGCGTAT	TCCACTACAAACCATTCGAGC	50.0	47.6	58.0	58.3	1523-1526 bp	1.0	70.1