Hotspot

accD-psaI

LSC · 58745-59741 · 997 bp

Back to Solidago

Pi 0.00573

MarkerSeek score 64.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 997
variable_sites: 5
pi: 0.00573
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAAGGCGTTCTGAGTGAGT	AAAGAAGCCATTGCGATTGC	45.0	45.0	57.4	58.0	1011-1135 bp	1.0	79.4
accD-psaI_p2	AAAGGCGTTCTGAGTGAGTT	AAAGAAGCCATTGCGATTGC	45.0	45.0	57.4	58.0	1010-1134 bp	1.0	79.4
accD-psaI_p3	AAAAGGCGTTCTGAGTGAGT	TTGCGATTGCCGGAAATACT	45.0	45.0	57.4	58.3	1001-1125 bp	1.0	77.4
accD-psaI_p4	AAAGGCGTTCTGAGTGAGTT	TTGCGATTGCCGGAAATACT	45.0	45.0	57.4	58.3	1000-1124 bp	1.0	77.4
accD-psaI_p5	AAAGGCGTTCTGAGTGAGTT	AGGCCTACTAAAGGGACCAA	45.0	50.0	57.4	57.6	980-1104 bp	1.0	76.6