Hotspot

trnE-rpoB

LSC · 11722-12756 · 1035 bp

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Pi 0.000971

MarkerSeek score 59.80

Primer available yes

Label trnE-rpoB

Feature Metadata

label_name: trnE-rpoB
feature_type: igs
region: LSC
length_bp: 1035
variable_sites: 1
pi: 0.000971
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-rpoB_p1	GGGACTTGAACCCTCGTTTT	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.0	56.8	1177 bp	1.0	62.5
trnE-rpoB_p2	TAACCACTGGACCATAGGGG	AGTTCTTCTGTCAAACCCTGA	55.0	42.9	58.1	56.8	1136 bp	1.0	61.8
trnE-rpoB_p3	CCCTCGTTTTCTCCGTGAAA	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.1	56.8	1167 bp	1.0	61.7
trnE-rpoB_p4	TTGAACCCTCGTTTTCTCCG	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.1	56.8	1172 bp	1.0	61.7
trnE-rpoB_p5	GGGACTTGAACCCTCGTTTT	TCAAACCCTGATCAATGAACCT	50.0	40.9	58.0	57.6	1167 bp	1.0	61.1